| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.059717 |
| Chromosome: | chromosome 3 |
| Location: | 2009742 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155850 | (1 of 1) IPR010912 - Spen paralogue/orthologue C-terminal, metazoa | 3'UTR | |
| Cre03.g155900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTATCACTCTCTCGTCTGTGCCTCTGTGGCCAATCACACGCGCTGTCGC |
| Internal bar code: | AGTTGCACACAATCCAGTTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3402 |
| LEAP-Seq percent confirming: | 98.0769 |
| LEAP-Seq n confirming: | 102 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 104 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTGGATGGGAGCAAGAC |
| Suggested primer 2: | GGGCACGTTCGAAAGGTCTA |