| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059720 |
| Chromosome: | chromosome 6 |
| Location: | 8887269 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311900 | RABG1,RAB7 | RAB-Like GTP binding Protein; (1 of 1) K07897 - Ras-related protein Rab-7A (RAB7A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCCTTGCAGACAGCTCTCAAGGCCCGAATCGCGTCACTCAGCGTCTG |
| Internal bar code: | GCCATGGTGGCTTTAGCTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4734 |
| LEAP-Seq percent confirming: | 71.2121 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 132 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAGGTGTCGGAGAAGAA |
| Suggested primer 2: | TGGATGAGAGTGCCTGCAAG |