| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059729 |
| Chromosome: | chromosome 7 |
| Location: | 3757013 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338300 | FBB14 | Putative flagellar/basal body protein; (1 of 1) PTHR10489//PTHR10489:SF612 - CELL ADHESION MOLECULE // BENT, ISOFORM F | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCCCGCCCGGTCATGCTCCTAGTCGATGCCCTGGCCGTCGGCCCAAT |
| Internal bar code: | CAGTTTCGAAGCTTCGTTGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3080 |
| LEAP-Seq percent confirming: | 90.4762 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTCGCATCTGATGTCGG |
| Suggested primer 2: | ACCGTACAAAGAGCCAGAGC |