| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059739 |
| Chromosome: | chromosome 7 |
| Location: | 4405530 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g343101 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGTTGCTTATATGCCGGTGCCGTATCGCAAATTCAAACTAACTAGTAT |
| Internal bar code: | ATGTTGTAACATTGGAGCGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1787 |
| LEAP-Seq percent confirming: | 32.4561 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 114 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCCGCCATTTCTTTT |
| Suggested primer 2: | CCTCGGGAGCCATTGTTTCT |