Insertion junction: CLIP2.059739_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.059739
Chromosome:chromosome 17
Location:4228623
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCGCGGCTCAGCCCACAGTGCTGTACCGTACTCCATGACCCCCAAGGC
Internal bar code:TTCGTTTCGTGGGACATGTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:3795
LEAP-Seq percent confirming:90.3226
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:GGACAACTCCACGTGGTACC
Suggested primer 2:AGGCTCTGAGGATTTTGGGC