| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059748 |
| Chromosome: | chromosome 10 |
| Location: | 5326407 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456700 | CASC3,EJC1 | (1 of 1) PF09405 - CASC3/Barentsz eIF4AIII binding (Btz); Exon Junction Complex component | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCACCACGATTTTCTTCACGCCACACATGGCGCCCGCGACGTCATGCA |
| Internal bar code: | CACCATCACACGAAAGAATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2568 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCGCGGATGAAGGCTAGT |
| Suggested primer 2: | TCAACTTCGCTTGCAACGTG |