| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059773 |
| Chromosome: | chromosome 10 |
| Location: | 6541221 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465500 | (1 of 1) 3.1.1.10 - Tropinesterase / Atropinesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTATTGCTCGTGACGTCAGGTTCCGGGTGTGCTGAGAGCTTTCCTTTG |
| Internal bar code: | GCATTTATGGCCGCCCATTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1942 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAATCCCGCCCACTAAGT |
| Suggested primer 2: | GTATGGGGTGGAGGTTGGTG |