| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.059781 |
| Chromosome: | chromosome 1 |
| Location: | 6068966 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042900 | RRM1 | Putative rRNA (adenine-N6-)-methyltransferase; (1 of 1) 2.1.1.183 - 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATTCACAGCTGTTACCTGCTTTTCCCTGCCGCACAGACTCGCTCGCT |
| Internal bar code: | TTAACTTAGTACGTTAGAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 777 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGCTTCCATTGGCAGATT |
| Suggested primer 2: | GCTCCAGGTTGTTACCCCTC |