Insertion junction: CLIP2.059791_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.059791
Chromosome:chromosome 10
Location:4342758
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g449800 (1 of 1) PF04657 - Putative inner membrane exporter, YdcZ (DMT_YdcZ) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTTTCGGTCTTGTCGATTTACCGAGTTTCCTCTTCACAAAGGATTTTT
Internal bar code:GTACTCTGAGAACACCGTTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:3006
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TGATGCAACGTTGGCTTGTG
Suggested primer 2:TTGTGGCTGCGGTAAGGTAG