| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.059794 |
| Chromosome: | chromosome 5 |
| Location: | 1221161 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241633 | (1 of 2) IPR006626//IPR011050//IPR012334 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor // Pectin lyase fold | 3'UTR | |
| Cre05.g800528 | (1 of 1) K11251//K13849 - histone H2A (H2A) // reticulocyte-binding protein (RBP) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATGGTGCAGGCCTGCAGCTGCTACTGTTCGTCGTGCGCTACAGCCTC |
| Internal bar code: | CAATAGATGCCCTGGAATCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCAGGTGTGTTTGTGTG |
| Suggested primer 2: | GTAACACACAGTGCAGCACG |