| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059802 |
| Chromosome: | chromosome 3 |
| Location: | 354406 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144627 | CGS1 | Cystathionine gamma-synthase; (1 of 1) 2.5.1.48 - Cystathionine gamma-synthase / O-succinylhomoserine synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAGCCCTGCCGCCGCACACTTCCGCACAGTCCGCGACGCCCACTTGTT |
| Internal bar code: | CAAAAAGGGGACTGTTTGTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3454 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGCTAACCGTAAGCAAC |
| Suggested primer 2: | CTCCCTCCCATACCAGGACA |