| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059834 |
| Chromosome: | chromosome 3 |
| Location: | 6423950 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192900 | OPR17 | (1 of 4) PTHR11584//PTHR11584:SF377 - SERINE/THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; OctotricoPeptide Repeat protein 17 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGATAGGAGCCCTGCCTCCACGAGTGCGACATGCCCCCACGGTATAAG |
| Internal bar code: | TGTTATTTATAGCGGTGAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3225 |
| LEAP-Seq percent confirming: | 97.3333 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACGCGAGACTATCATGCC |
| Suggested primer 2: | TGTCAATAAGGGCCCGCAAT |