| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.059850 |
| Chromosome: | chromosome 9 |
| Location: | 439928 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749447 | AHD1 | (1 of 2) K12524 - bifunctional aspartokinase / homoserine dehydrogenase 1 (thrA); Bifunctional aspartate kinase/homoserine dehydrogenase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGTCAACGGCACGGTGCGATCAACCCAGGGTGTAGGCACAAGAAATG |
| Internal bar code: | CGTGGTTGCGAGTGATGCTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3051 |
| LEAP-Seq percent confirming: | 41.1765 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCGCTATGAGAGTGTCCC |
| Suggested primer 2: | CACGCTTCCTTATGCCATGC |