Insertion junction: CLIP2.059872_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.059872
Chromosome:chromosome 3
Location:6355998
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g192400 (1 of 1) IPR013769 - Band 3 cytoplasmic domain 3'UTR_intron
Cre03.g192450 CYA3 Adenylate cyclase; (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTAACATGGCCCTTTAATCCTCCACACCGCAACATTTATTTGCAGGAC
Internal bar code:CTTAAAAATACCCATTCCTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1990
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAGGAGACATTCGATCCG
Suggested primer 2:CCGCCTCTACCTTTCGATCC