| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059875 |
| Chromosome: | chromosome 8 |
| Location: | 3241558 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377050 | (1 of 1) 4.6.1.14 - Glycosylphosphatidylinositol diacylglycerol-lyase / VSG-lipase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCCGTTCAGTAGTGCCAAATCCAGGACACGTCCCCACAGCTGATGAC |
| Internal bar code: | ACCATAGATTGAGTGGTAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1225 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCTGTAGCTGCAGGTAG |
| Suggested primer 2: | TCACTGCAAGGCGCTTAGAA |