Insertion junction: CLIP2.059882_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.059882
Chromosome:chromosome 3
Location:3062853
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g163900 PWR3 (1 of 14) PF04720 - PDDEXK-like family of unknown function (PDDEXK_6); PWR motif protein 3'UTR
Cre03.g163950 CDO2 (1 of 2) 1.13.11.20 - Cysteine dioxygenase; Cysteine dioxygenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGACAGTGCCCTCCCAGCTCAAGACGCACGAGCAGACATGGCCACATGC
Internal bar code:CGATATGTATATAACTTTATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1459
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGGCTGGGTTGATAACG
Suggested primer 2:AGGCTGAGGCGCAATAAACT