Insertion junction: CLIP2.059888_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.059888
Chromosome:chromosome 12
Location:5528415
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g530450 (1 of 4) IPR001841//IPR018957 - Zinc finger, RING-type // Zinc finger, C3HC4 RING-type outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCAGCCCGCTGTGTTCATGCGATGGTCCATGGATGCGCAGGCGAAGGT
Internal bar code:CGAATTATCTACTGGTATGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3403
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:89
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:89

Suggested primers for confirmation by PCR

Suggested primer 1:CTGAGTTGGACCAGGAGCAG
Suggested primer 2:CGGACCTGCTTTTTGTTGGG