| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059888 |
| Chromosome: | chromosome 12 |
| Location: | 5530311 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530450 | (1 of 4) IPR001841//IPR018957 - Zinc finger, RING-type // Zinc finger, C3HC4 RING-type | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAACAGGAATACATGCAATCAACTTACGATGCTACGAAACAACAGCT |
| Internal bar code: | AGCGTAGGCCTGCATCGGCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3159 |
| LEAP-Seq percent confirming: | 30.4878 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACCTGCTTTTTGTTGGG |
| Suggested primer 2: | CTGAGTTGGACCAGGAGCAG |