| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.059893 |
| Chromosome: | chromosome 6 |
| Location: | 5104109 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282750 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGCATGCTCACTACGTACTCAGCTCACTACAGAACTGAAATGGTGACG |
| Internal bar code: | CATGGGGGCGGTTTCGTAGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2244 |
| LEAP-Seq percent confirming: | 44.5545 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 101 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTCGCTTTTGCCTCCAGC |
| Suggested primer 2: | ATTGAGGGAGCAGTGAAGGC |