| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059912 |
| Chromosome: | chromosome 10 |
| Location: | 5398517 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801181 | 5'UTR_intron | ||
| Cre10.g801182 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGGTGAGTGACGGCGGGCACGCGTGCGGCGTGCCGACGCGCCACAAG |
| Internal bar code: | TTTGATCGCTGCATGCGGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4485 |
| LEAP-Seq percent confirming: | 47.7273 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGAGTAGCAGCTACGGC |
| Suggested primer 2: | ATCACGAAGGCGGAGTTTGT |