Insertion junction: CLIP2.059928_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.059928
Chromosome:chromosome 1
Location:87511
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g000550 Putative nucleotide-diphospho-sugar transferase; (1 of 6) PTHR10994//PTHR10994:SF61 - RETICULON // RETICULON-LIKE PROTEIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATCACAACGGGAACTCGTCTTTCATCCCCTCTGCTCTTGCTTGTCCTA
Internal bar code:AGGATTTTACCCGGAAACGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:204
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GACCTGTTACGGTCCCTGTG
Suggested primer 2:CCGAGTCTCTTGCTGTGTGT