| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059935 |
| Chromosome: | chromosome 3 |
| Location: | 2539449 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159700 | (1 of 1) PF04750 - FAR-17a/AIG1-like protein (Far-17a_AIG1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGCTTTAAATCGCAAGCGCAAACCCCAGCTTCCTCTGCCAAAGTTTG |
| Internal bar code: | GCGGATACAGACTAAGGGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5039 |
| LEAP-Seq percent confirming: | 99.2647 |
| LEAP-Seq n confirming: | 135 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 136 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGGGCATTCACGTCCTA |
| Suggested primer 2: | TGTACCCGTTCTTGGACACG |