| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059957 |
| Chromosome: | chromosome 11 |
| Location: | 1813373 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467550 | (1 of 1) 3.5.2.3 - Dihydroorotase / DHOase; Dihydroorotase/amidohydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTCCTATCTTAATGTCTCCAGACATTAATTGGCCATTTTGGCCGTTT |
| Internal bar code: | AGGATGGGTGAGATATGGTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCGACAAGCTGACTCTG |
| Suggested primer 2: | CCTCCCTTGCCATGTTCCAT |