| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.059969 |
| Chromosome: | chromosome 14 |
| Location: | 3989952 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633100 | (1 of 1) PF12070 - Protein of unknown function (DUF3550/UPF0682) (DUF3550) | 5'UTR | |
| Cre14.g633150 | OPR66 | OctotricoPeptide Repeat protein, probably a pseudogene | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCCAGATTTAGAATTGGGCGACAAAGATGGCCTATGTCACCGTGAGC |
| Internal bar code: | TGTCGAAAGTGTACTCACAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1438 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTCTCGCAAATTCGCTG |
| Suggested primer 2: | ACATCGGACTGGCTTGTGAG |