| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059970 |
| Chromosome: | chromosome 13 |
| Location: | 3768925 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588800 | (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGATGACATGCATGCAGGGGTCTGGGCCCAAATGGTCTGGGCCAGGGCC |
| Internal bar code: | ATTTTTGTTCATTCGAATTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2341 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGGGATGAAACGTGGAG |
| Suggested primer 2: | GCGGTCGTGCAGATATCTCA |