| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059972 |
| Chromosome: | chromosome 7 |
| Location: | 6055167 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355200 | ORC5 | Origin recognition complex subunit 5; (1 of 1) PTHR12705//PTHR12705:SF0 - ORIGIN RECOGNITION COMPLEX SUBUNIT 5 // ORIGIN RECOGNITION COMPLEX SUBUNIT 5 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACGTAGAACGGGGTGTCCCAGGGCCACCCAACTGGGGGAACAGTGGG |
| Internal bar code: | AATAGCGATTTTGAGGTGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2557 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCCTGGTGGAATTCAGTC |
| Suggested primer 2: | GGCCCCTTGACTTTATGGCT |