| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059976 |
| Chromosome: | chromosome 16 |
| Location: | 7587479 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684267 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGATTGTAGGCCCATGCGCAGTGGGTGGGCAGTCTCCCCCCCTGAAAGA |
| Internal bar code: | GATCTATAATTTCTGTACAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1929 |
| LEAP-Seq percent confirming: | 99.0826 |
| LEAP-Seq n confirming: | 108 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGTCGTCAGGCTTCTTT |
| Suggested primer 2: | ATGATGAAGACGACCGCTCC |