| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.059978 |
| Chromosome: | chromosome 6 |
| Location: | 8311916 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307200 | (1 of 1) PTHR10358 - ENDOSULFINE | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGCGCCCCCCCATTCCCTGCCAAGCTGCTCTACTTGTCAAGGGGGTC |
| Internal bar code: | CGCGATGTGTATAATGGAATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2976 |
| LEAP-Seq percent confirming: | 98.5916 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATGGCAATACGCATGCG |
| Suggested primer 2: | GTGAAAGGGGTACGTGTCGT |