| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059981 |
| Chromosome: | chromosome 9 |
| Location: | 5556648 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409350 | RLI1 | (1 of 1) K06174 - ATP-binding cassette, sub-family E, member 1 (ABCE1); Fe-S ABC transporter | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGTGCAGAGTGTCTAGGCCGGGTCACCAGGTCTGCAGGGAAGCACAC |
| Internal bar code: | GTCCAATAAGGAATGAAAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 820 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACTCCTGACGACACTTC |
| Suggested primer 2: | CAGCAGGAGCAGATATGCCA |