Insertion junction: CLIP2.059988_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.059988
Chromosome:chromosome 3
Location:1025800
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g148150 (1 of 5) PF04884 - Vitamin B6 photo-protection and homoeostasis (DUF647) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTACGCACGTGAACGCGCGCGGTGACCACCGCAGGTAAATTGGGCGAG
Internal bar code:CGGATAATCACAGTTGTCCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:732
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:AAATGCTGCAACGCTCTGTG
Suggested primer 2:AATGCCAGACAAAAGCAGCG