| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059995 |
| Chromosome: | chromosome 1 |
| Location: | 336616 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001800 | MLCK1,FAP403 | (1 of 4) PTHR24361//PTHR24361:SF411 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED; Flagellar Associated Protein 403 | 3'UTR |
| Cre01.g001950 | Predicted protein with 3-phosphoshikimate1-carboxyvinyltransferase core domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCAGGCCACCTTTTACTCCATCCAGGCCCCACCATCCGAGGTTCTAG |
| Internal bar code: | AGCGTCAGGATACGGATAGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 288 |
| LEAP-Seq percent confirming: | 23.0769 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTTGACAAACACGCATT |
| Suggested primer 2: | CCCGTGGACTGTTCGTAACA |