| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059996 |
| Chromosome: | chromosome 17 |
| Location: | 4523492 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g731466 | (1 of 25) IPR000210//IPR011333 - BTB/POZ domain // POZ domain | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTCGCTGGGCCAGTATTAACAACATTCCCCCCTCCTTTCCCACTTCC |
| Internal bar code: | TACTATGACAGGCGCGTTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1408 |
| LEAP-Seq percent confirming: | 81.4815 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCAACACCAACGTGCAGG |
| Suggested primer 2: | GAGAAGCACGGACTGACACA |