| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.060004 |
| Chromosome: | chromosome 7 |
| Location: | 5131612 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348350 | PWR8 | (1 of 14) PF04720 - PDDEXK-like family of unknown function (PDDEXK_6); PWR motif protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTACAGAGCTGCAGACATCTGCACACATGTAAACGCCGCCGGCCCAAC |
| Internal bar code: | GCCCATGCCAGGTATTCATAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 795 |
| LEAP-Seq percent confirming: | 46.6667 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTTCTGAAGCAGGGTCTT |
| Suggested primer 2: | CCAACAGCAACTGTCAACGG |