Insertion junction: CLIP2.060035_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.060035
Chromosome:chromosome 2
Location:7660469
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g147500 3'UTR
Cre02.g147550 (1 of 2) IPR009057//IPR017877 - Homeodomain-like // Myb-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATAACCCACTACCAAGCAACTCTACCCAGGCACTTGGCCACCCGCAGCC
Internal bar code:GGTCTGCTGTTGTCCGTGCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:601
LEAP-Seq percent confirming:96.7742
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCGCTTGTGATGTACATG
Suggested primer 2:GTTAGCTTTAGACGCACGCG