| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060046 |
| Chromosome: | chromosome 6 |
| Location: | 6327096 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293000 | PRI2,DIV37 | (1 of 1) K02685 - DNA primase large subunit (PRI2); Probable large subunit of DNA primase. | 3'UTR |
| Cre06.g800716 | (1 of 1) K14015 - nuclear protein localization protein 4 homolog (NPLOC4, NPL4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAAGCGGTGCGCGTGCGCAAACCCAATCATTCCCATGACGCTGTATG |
| Internal bar code: | GTTTTGCATGTGTAAGTAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGACCCGAACGATATGG |
| Suggested primer 2: | CGCTGGTCGACATGTGTTTG |