Insertion junction: CLIP2.060050_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.060050
Chromosome:chromosome 12
Location:3875851
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g515250 ROC7 Rythm Of Chloroplast bioluminescence 7; (1 of 1) PF09758 - Uncharacterised conserved protein (FPL) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACGCCATCACGCAACTCGCCCCGGACCGTCAAGTACTCGTACACAACG
Internal bar code:AGTGTGAGGCCTACCTGCGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1816
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CCACACCGGAACTGTGATCA
Suggested primer 2:GAACAACGCACCTGACAACC