Insertion junction: CLIP2.060050_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.060050
Chromosome:chromosome 12
Location:3875853
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g515250 ROC7 Rythm Of Chloroplast bioluminescence 7; (1 of 1) PF09758 - Uncharacterised conserved protein (FPL) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGTGGCGGACGCTCCAGGGAGGGGGTGGTGGACTGGCGGGGTATGTGG
Internal bar code:AGTGTGAGGCCTACCTGCGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2665
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GAACAACGCACCTGACAACC
Suggested primer 2:CCACACCGGAACTGTGATCA