| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.060052 |
| Chromosome: | chromosome 17 |
| Location: | 5396837 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738550 | (1 of 1) K00774 - poly [ADP-ribose] polymerase 16 (PARP16) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGCACTCCCGTCATCGCAAGCGCCCGGGCTCTCAAGATGGTTGAGTC |
| Internal bar code: | AGTGCGCAATTACAGCGACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 179 |
| LEAP-Seq percent confirming: | 31.25 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTTTGTCCCAGCCCAAA |
| Suggested primer 2: | GCCGCAAAACCGAACTTGAT |