| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.060060 |
| Chromosome: | chromosome 10 |
| Location: | 5399233 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801181 | 5'UTR_intron | ||
| Cre10.g801182 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGAGTTTGTTAAGTACATAGGGCGCATGCTGGGCGCGTTCCGGGAGAA |
| Internal bar code: | ACAGCGTCCATTAACTAAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3045 |
| LEAP-Seq percent confirming: | 1.5625 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACCCTCAGCGAAACTTC |
| Suggested primer 2: | CGTTGGGAGCTCATGACTGT |