| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060066 |
| Chromosome: | chromosome 4 |
| Location: | 1127938 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215900 | (1 of 2) IPR006212//IPR009030//IPR011641 - Furin-like repeat // Insulin-like growth factor binding protein, N-terminal // Tyrosine-protein kinase ephrin type A/B receptor-like | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCACGCACGTTCGCACTTGAGCAAAACCCAGTTCGCCTGACGCGCTG |
| Internal bar code: | TGCGTTCCTACCATAATGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGCATAGTTGACCATGC |
| Suggested primer 2: | CACAGTCACGAACGTCTGGA |