| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.060074 |
| Chromosome: | chromosome 2 |
| Location: | 7707426 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g147050 | (1 of 7) PF05517 - p25-alpha (p25-alpha); P25 Alpha Domain Containing Flagellar Associated Protein | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGATGGGATCTGGGGCACTGACCTTGCGTTTGCTGTAACGAGGGCCAT |
| Internal bar code: | TTGGTGAATGCCAATGGACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1573 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGCAGCAGTGACAGCAG |
| Suggested primer 2: | GCCTTCCGTACTGACACACA |