| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060078 |
| Chromosome: | chromosome 7 |
| Location: | 2514656 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329700 | NSG9,RPT2 | 26S proteasome regulatory subunit; (1 of 1) K03062 - 26S proteasome regulatory subunit T2 (PSMC1, RPT2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATGTGAGCGCATCTTACCACATCTCATCCACACGCGCGCGCAGGGGT |
| Internal bar code: | TTTCTTCCTGGTGTGCCCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3352 |
| LEAP-Seq percent confirming: | 90.8257 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGAAGAAGGAGGGCGTG |
| Suggested primer 2: | TCCGCAGTTGCTGAAGTCAT |