| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060086 |
| Chromosome: | chromosome 9 |
| Location: | 497459 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g748997 | (1 of 3) PTHR11132//PTHR11132:SF64 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED | 3'UTR | |
| Cre18.g749047 | (1 of 3) 2.4.1.68 - Glycoprotein 6-alpha-L-fucosyltransferase / Guanosine diphosphofucose-glycoprotein fucosyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCCCATCATGTTTGCACTTCTGGGCAAGCGGGCGAATGTCGTACTTC |
| Internal bar code: | CTAGTGCCCAGATGAAGTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1651 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTGTACAAACCGACCTC |
| Suggested primer 2: | TGTCACGGTGTAGCACAGAC |