| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060090 |
| Chromosome: | chromosome 6 |
| Location: | 3333731 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGGTAAAAATGCTGCCACCGGCGGCGCTGGAGGCATTGCTGGCGTCGG |
| Internal bar code: | CAATCATTGTTTTTTATGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 3.7037 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATAGAACGCGCTCTTCCG |
| Suggested primer 2: | GATCCTGTCTGATGGCTCGG |