Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.060099 |
Chromosome: | chromosome 9 |
Location: | 5248455 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g407100 | MRPS5,uS5m | Mitochondrial ribosomal protein S5; (1 of 1) PTHR13718:SF61 - 28S RIBOSOMAL PROTEIN S5, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATTGATGCGGGGCCTGGGGCGGCTGTCACTGTCGGTGGGGGGGTACT |
Internal bar code: | TAGCTTTTCCTAAAAACTACTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1472 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 21 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGACAGGTCCAGCTCTTGC |
Suggested primer 2: | AATGCCAACGAAGGGGTAGG |