Insertion junction: CLIP2.060122_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.060122
Chromosome:chromosome 12
Location:7867573
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g553600 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTTCCTACCCCCCCCCTGGCGTTTCCCACACTTCGACCTTCATAACAA
Internal bar code:TCCTCTGTATCCAAAGACTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1563
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAGAGGGATTGTGCACTT
Suggested primer 2:CCAAAGTCCAGGGCAGACAT