Insertion junction: CLIP2.060151_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.060151
Chromosome:chromosome 6
Location:3806479
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278175 (1 of 1) K14793 - ribosomal RNA-processing protein 9 (RRP9) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCACCTACACCCACATCCACCTCACATCCTAACACGCACAGGCGCAGC
Internal bar code:TCCATACGCAAAGCTGGGAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1973
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAGAGATGGGGAAGGGAG
Suggested primer 2:CGCAGAAGGAGGATGCAAGA