| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.060153 |
| Chromosome: | chromosome 3 |
| Location: | 8938084 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204577 | DNJ31 | (1 of 1) IPR000104//IPR001623//IPR003072 - Antifreeze protein, type I // DnaJ domain // Orphan nuclear receptor, NOR1 type; DnaJ-like protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAAGGGTTCGTGGCGGTTGGATGCACGCGCAAGCGTCTATGACCTAA |
| Internal bar code: | TAGTCATCACGGGGGATCATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1829 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGGTAAACGAGCAGGCA |
| Suggested primer 2: | CTTGCCCCTATACCCTCCCT |