| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060154 |
| Chromosome: | chromosome 16 |
| Location: | 5955112 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678900 | HIK,HKR6,HIK6 | (1 of 3) IPR001789//IPR011006 - Signal transduction response regulator, receiver domain // CheY-like superfamily; Response regulator of two-component system | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCTGTGACAAATTCATCTTCTCATCATTTCATGGCGACTGCGCACCG |
| Internal bar code: | CTCTCTGTGGGCGTTCGAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5728 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 144 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 144 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCAGTACGGTTTTTGCA |
| Suggested primer 2: | GGAGTTCGGACAGTGGACAG |