| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060168 |
| Chromosome: | chromosome 10 |
| Location: | 4351864 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449901 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAAAGCCGCGCCAATTGGCATCACGTGAAGGCTATCCACCAAAACCG |
| Internal bar code: | GATAGGGTAATGGACGCTTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1410 |
| LEAP-Seq percent confirming: | 3.44828 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCGCTCGGTATTAGAC |
| Suggested primer 2: | CGTCAACTGAGCACAGACCT |